High-sensitivity chemiluminescence detection of cytokines using an antibody-immobilized CMOS image sensor

Dong-Gu Hong; Hyou-Arm Joung; Sang-Hyo Kim; Min-Gon Kim

doi:10.1117/12.2018701

20 May 2013 High-sensitivity chemiluminescence detection of cytokines using an antibody-immobilized CMOS image sensor

Dong-Gu Hong, Hyou-Arm Joung, Sang-Hyo Kim, Min-Gon Kim

Proceedings Volume 8879, Nano-Bio Sensing, Imaging, and Spectroscopy; 88790O (2013) https://doi.org/10.1117/12.2018701
Event: Nano-Bio Sensing, Imaging and Spectroscopy, 2013, Jeju, Korea, Republic of

Abstract

In this study, we used a Complementary Metal Oxide Semiconductor (CMOS) image sensor with immobilizing antibodies on its surface to detect human cytokines, which are activators that mediate intercellular communication including expression and control of immune responses. The CMOS image sensor has many advantages over the Charge Couple Device, including lower power consumption, operation voltage, and cost. The photodiode, a unit pixel component in the CMOS image sensor, receives light from the detection area and generates digital image data. About a million pixels are embedded, and size of each pixel is 3 x 3 μm. The chemiluminescence reaction produces light from the chemical reaction of luminol and hydrogen peroxide. To detect cytokines, antibodies were immobilized on the surface of the CMOS image sensor, and a sandwich immunoassay using an HRP-labeled antibody was performed. An HRP-catalyzed chemiluminescence reaction was measured by each pixel of the CMOS image sensor. Pixels with stronger signals indicated higher cytokine concentrations; thus, we were able to measure human interleukin-5 (IL-5) at femtomolar concentrations.

Citation Download Citation

Dong-Gu Hong, Hyou-Arm Joung, Sang-Hyo Kim, and Min-Gon Kim "High-sensitivity chemiluminescence detection of cytokines using an antibody-immobilized CMOS image sensor", Proc. SPIE 8879, Nano-Bio Sensing, Imaging, and Spectroscopy, 88790O (20 May 2013); https://doi.org/10.1117/12.2018701

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