Molecular sensing for biomarkers of invasive cancer cells using localized surface plasmon resonance

Yoochan Hong; Minhee Ku; Jin-Suck Suh; Yong-Min Huh; Dae Sung Yoon; Jaemoon Yang

doi:10.1117/12.2018326

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20 May 2013 Molecular sensing for biomarkers of invasive cancer cells using localized surface plasmon resonance

Yoochan Hong, Minhee Ku, Jin-Suck Suh, Yong-Min Huh, Dae Sung Yoon, Jaemoon Yang

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Proceedings Volume 8879, Nano-Bio Sensing, Imaging, and Spectroscopy; 88790Z (2013) https://doi.org/10.1117/12.2018326
Event: Nano-Bio Sensing, Imaging and Spectroscopy, 2013, Jeju, Korea, Republic of

Abstract

In this study, we describe the development of cancer biomarker-sensitive nanobiosensor based on localized surface plasmon resonance (LSPR) that enabling recognition for proteolytic activity of membrane type 1 matrix metalloproteinase (MT1-MMP) anchored on invasive cancer cells. First of all, we prepared biomarker-detectable substrate based on gold nanorods (GNRs) using nanoparticle adsorption method. The sensitivity of sensing chip was confirmed using various solvents that having different refractive indexes. Subsequently, MT1-MMP-specific cleavable peptide was conjugated onto surface of GNRs and molecular sensing about proteolytic activity was conducted using MT1-MMP and cell lysates. Collectively, we developed biomarker detectable sensor, which allows for the effective detection of proteolytic activity about MT1-MMP extracted from invasive cancer cells.

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Yoochan Hong, Minhee Ku, Jin-Suck Suh, Yong-Min Huh, Dae Sung Yoon, and Jaemoon Yang "Molecular sensing for biomarkers of invasive cancer cells using localized surface plasmon resonance", Proc. SPIE 8879, Nano-Bio Sensing, Imaging, and Spectroscopy, 88790Z (20 May 2013); https://doi.org/10.1117/12.2018326

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