Synthesis and characterization of nano ceria for biological applications

Hsueh-Shih Chen; Julie Mazzolini; Jon Ayers; Josh Rappoport; Jamie Lead; Jon Preece

doi:10.1117/12.2018564

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20 May 2013 Synthesis and characterization of nano ceria for biological applications

Hsueh-Shih Chen, Julie Mazzolini, Jon Ayers, Josh Rappoport, Jamie Lead, Jon Preece

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Proceedings Volume 8879, Nano-Bio Sensing, Imaging, and Spectroscopy; 887910 (2013) https://doi.org/10.1117/12.2018564
Event: Nano-Bio Sensing, Imaging and Spectroscopy, 2013, Jeju, Korea, Republic of

Abstract

We have synthesized ceria nanoparticles by reacting cerium salts and sodium hydroxide without any additive in order to avoid possible cytotoxicity. The synthesized ceria nanoparticles were with cubic structure according to X-ray diffraction and have diameters between 6 and 10 nm analyzed by transmission electron microscopy. Aggregation behaviors of ceria nanoparticles in DI water and cell culture media have been studied by dynamic light scattering. In de-ionized water, ceria nanoparticles formed a stable colloidal solution with hydrodynamic size ~ 70 nm and zeta potential ~ 47 mV. Dispersion of the nanoparticles in serum-free media was found to result in larger aggregates, while the nanoparticles in serumcontaining media form a stable solution, which suggests that proteins in serum and ceria nanoparticles have some interactions. In cellular uptake test, entry of ceria nanoparticles into HeLa cells incubated in serum-containing media at 37 °C for 60 min was observed and without cytotoxicity.

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Hsueh-Shih Chen, Julie Mazzolini, Jon Ayers, Josh Rappoport, Jamie Lead, and Jon Preece "Synthesis and characterization of nano ceria for biological applications", Proc. SPIE 8879, Nano-Bio Sensing, Imaging, and Spectroscopy, 887910 (20 May 2013); https://doi.org/10.1117/12.2018564

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