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20 May 2013Synthesis and characterization of nano ceria for biological applications
We have synthesized ceria nanoparticles by reacting cerium salts and sodium hydroxide without any additive in order to
avoid possible cytotoxicity. The synthesized ceria nanoparticles were with cubic structure according to X-ray diffraction
and have diameters between 6 and 10 nm analyzed by transmission electron microscopy. Aggregation behaviors of ceria
nanoparticles in DI water and cell culture media have been studied by dynamic light scattering. In de-ionized water, ceria
nanoparticles formed a stable colloidal solution with hydrodynamic size ~ 70 nm and zeta potential ~ 47 mV. Dispersion
of the nanoparticles in serum-free media was found to result in larger aggregates, while the nanoparticles in serumcontaining
media form a stable solution, which suggests that proteins in serum and ceria nanoparticles have some
interactions. In cellular uptake test, entry of ceria nanoparticles into HeLa cells incubated in serum-containing media at
37 °C for 60 min was observed and without cytotoxicity.
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Hsueh-Shih Chen, Julie Mazzolini, Jon Ayers, Josh Rappoport, Jamie Lead, Jon Preece, "Synthesis and characterization of nano ceria for biological applications," Proc. SPIE 8879, Nano-Bio Sensing, Imaging, and Spectroscopy, 887910 (20 May 2013); https://doi.org/10.1117/12.2018564