Quantitative fluorescence molecular imaging in highly light-absorbing melanomas using a dual-tracer kinetic modeling normalization method

Kenneth M. Tichauer; Stephen C. Kanick; Sophie J. Deharvengt; Kimberley S. Samkoe; Tayyaba Hasan; Radu V. Stan; Brian W. Pogue

doi:10.1117/12.2037424

4 March 2014 Quantitative fluorescence molecular imaging in highly light-absorbing melanomas using a dual-tracer kinetic modeling normalization method

Kenneth M. Tichauer, Stephen C. Kanick, Sophie J. Deharvengt, Kimberley S. Samkoe, Tayyaba Hasan, Radu V. Stan, Brian W. Pogue

Author Affiliations +

Proceedings Volume 8926, Photonic Therapeutics and Diagnostics X; 89260T (2014) https://doi.org/10.1117/12.2037424
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Tissues with high light absorption, such as melanomas, present a significant challenge to fluorescence imaging approaches that seek to estimate molecular expression in vivo, since any fluorescence originating in the tissue will suffer substantial attenuation prior to detection. This can lead to sizable underestimations in estimated fluorescent tracer concentration in these tissues using conventional fluorescence imaging. In this study, a dual-tracer fluorescence imaging approach was employed to correct for severe tissue absorption by 1) using simultaneous injection and imaging of an untargeted tracer to normalize tissue absorption effects on the targeted tracer, and 2) using kinetic modeling that capitalizes on subtle differences in the dynamics of targeted and untargeted tracer uptake to quantify targeted molecule concentrations in the high absorbing tissue. Monte Carlo simulation and kinetic models demonstrated that the effect of optical properties on the approach could be eliminated by a pixel-by-pixel normalization of the targeted and untargeted tracer uptakes prior to 5 min post-tracer injection for fluorescence planar dynamic imaging.

Citation Download Citation

Kenneth M. Tichauer, Stephen C. Kanick, Sophie J. Deharvengt, Kimberley S. Samkoe, Tayyaba Hasan, Radu V. Stan, and Brian W. Pogue "Quantitative fluorescence molecular imaging in highly light-absorbing melanomas using a dual-tracer kinetic modeling normalization method", Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X, 89260T (4 March 2014); https://doi.org/10.1117/12.2037424

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available