Interpreting CARS images of tissue within the C-H-stretching region

Benjamin Dietzek; Tobias Meyer; Anna Medyukhina; Norbert Bergner; Christoph Krafft; Bernd F. M. Romeike; Rupert Reichart; Rolf Kalff; Michael Schmitt; Jürgen Popp

doi:10.1117/12.2036547

20 March 2014 Interpreting CARS images of tissue within the C-H-stretching region

Benjamin Dietzek, Tobias Meyer, Anna Medyukhina, Norbert Bergner, Christoph Krafft, Bernd F. M. Romeike, Rupert Reichart, Rolf Kalff, Michael Schmitt, Jürgen Popp

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Proceedings Volume 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 892806 (2014) https://doi.org/10.1117/12.2036547
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Single band coherent anti-Stokes Raman scattering (CARS) microscopy within the CH-stretching region is applied to detect individual cells and nuclei of human brain tissue and brain tumors – an information which allows for histopathologic grading of the tissue. The CARS image contrast within the C-H-stretching region correlated to the tissue composition. Based on the specific application example of identifying nuclei within (coherent) Raman images of neurotissue sections, we shall derive general design parameters for lasers optimally suited to serve in a clinical environment and discuss the potential of recently developed methods to analyze spectrally resolved CARS images and image segmentation algorithms.

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Benjamin Dietzek, Tobias Meyer, Anna Medyukhina, Norbert Bergner, Christoph Krafft, Bernd F. M. Romeike, Rupert Reichart, Rolf Kalff, Michael Schmitt, and Jürgen Popp "Interpreting CARS images of tissue within the C-H-stretching region", Proc. SPIE 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 892806 (20 March 2014); https://doi.org/10.1117/12.2036547

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KEYWORDS

Tissues

Microscopy

Molecules

Proteins

Raman spectroscopy

CARS tomography

Brain

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