Photothermal therapy of human glioma spheroids with gold-silica nanoshells and gold nanorods: a comparative study

Suyog Chhetri; Henry Hirschberg M.D.; Steen J. Madsen

doi:10.1117/12.2038233

5 March 2014 Photothermal therapy of human glioma spheroids with gold-silica nanoshells and gold nanorods: a comparative study

Suyog Chhetri, Henry Hirschberg M.D., Steen J. Madsen

Author Affiliations +

Proceedings Volume 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 89280U (2014) https://doi.org/10.1117/12.2038233
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The efficacy of gold-silica nanoshells (AuNS) and gold nanorods (AuNR) for photothermal therapy was investigated in an in vitro system consisting of hybrid murine macrophage/human glioma spheroids. Macrophages were used as delivery vectors for the nanoparticles. Hybrid spheroids were formed via centrifugation of human glioma cells and nanoparticleloaded macrophages. Forty-eight hours post-centrifugation, the resultant 400 μm dia. spheroids were exposed to 808 nm laser light for 10 min. at irradiances ranging from 2 - 28 W cm^-2. Treatment efficacy was evaluated from spheroid growth kinetics over a 14-day period. AuNS were shown to have greater efficacy compared to AuNR. For example, hybrid spheroids consisting of a 5:1 ratio of glioma cells to AuNS-loaded macrophages exhibited significant growth inhibition when subjected to irradiances of 7 W cm^-2. In contrast, no growth inhibition was observed for the AuNR-macrophage hybrid spheroids, even at the highest irradiance investigated (28 W cm^-2). Growth inhibition was observed at 28 W cm^-2 when the nanorod concentration was increased, i.e., by forming hybrid spheroids with a 2:1 ratio of glioma cells to macrophages.

Citation Download Citation

Suyog Chhetri, Henry Hirschberg M.D., and Steen J. Madsen "Photothermal therapy of human glioma spheroids with gold-silica nanoshells and gold nanorods: a comparative study", Proc. SPIE 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 89280U (5 March 2014); https://doi.org/10.1117/12.2038233

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