Activity-dependent signal changes in neurons by fiber-coupled microscopy

Takashi Sakurai; Kowa Koida

doi:10.1117/12.2038407

5 March 2014 Activity-dependent signal changes in neurons by fiber-coupled microscopy

Takashi Sakurai, Kowa Koida

Proceedings Volume 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 89280V (2014) https://doi.org/10.1117/12.2038407
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

To study neuronal functions in brain, we developed a higher resolution type fiber-coupled microscope (FCM), and measured the activity-dependent fluorescence intensity of the excitable cells over time. FCM was constructed by combining a fluorescence microscope with the high density type of fiber bundle, which consisted of 1.5 x 10⁴ unit fiber in the assemble less than 0.5 mm tip. The spatial resolution was calculated to be 2.4 mm with the 5 mm focal depth. The activity-dependent Ca signals were detectable in each cell of either the pancreatic spheroids or the brain slices. The present FCM is very promising for detailed studies with the live imaging of signal molecules in the body at a single cell level.

Citation Download Citation

Takashi Sakurai and Kowa Koida "Activity-dependent signal changes in neurons by fiber-coupled microscopy", Proc. SPIE 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 89280V (5 March 2014); https://doi.org/10.1117/12.2038407

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