Biconically tapered fiber optic dip probe for rapid label-free immunoassays

John Miller; Angelica Castaneda; Kun Ho Lee; Martin Sanchez; Shelton Murinda; Wei-Jen Lin; Ertan Salik

doi:10.1117/12.2037987

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20 February 2014 Biconically tapered fiber optic dip probe for rapid label-free immunoassays

John Miller, Angelica Castaneda, Kun Ho Lee, Martin Sanchez, Shelton Murinda, Wei-Jen Lin, Ertan Salik

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Proceedings Volume 8938, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV; 893815 (2014) https://doi.org/10.1117/12.2037987
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

We report U-shaped biconically tapered optical fibers (BTOF) as dip probes for label-free immunoassays. The tapered regions of the sensors were functionalized by immobilization of immunoglobulin-G (Ig-G) and tested for detection of anti-IgG at concentrations of 0.5, 5.0, and 50 μg/mL. Antibody-antigen reaction creates a biological nanolayer modifying the waveguide structure leading to a change in the sensor signal, which allows real-time monitoring. The kinetics of the antibody (mouse Ig-G) -antigen (rabbit anti-mouse IgG) reactions was studied. The limit of detection for the sensor was estimated to be less than 0.5 μg/mL with low temperature sensitivity. Utilization of the rate of the sensor peak shift within the first few minutes of antibody-antigen reaction is proposed as a rapid detection method.

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John Miller, Angelica Castaneda, Kun Ho Lee, Martin Sanchez, Shelton Murinda, Wei-Jen Lin, and Ertan Salik "Biconically tapered fiber optic dip probe for rapid label-free immunoassays", Proc. SPIE 8938, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV, 893815 (20 February 2014); https://doi.org/10.1117/12.2037987

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