Real-time depth-resolved fiber optic Raman endoscopy for in vivo diagnosis of gastric precancer

Mads Sylvest Bergholt; Wei Zheng; Khek Yu Ho; Khay Guan Yeoh; Ming Teh; Jimmy Bok Yan So; Zhiwei Huang

doi:10.1117/12.2039552

4 March 2014 Real-time depth-resolved fiber optic Raman endoscopy for in vivo diagnosis of gastric precancer

Mads Sylvest Bergholt, Wei Zheng, Khek Yu Ho, Khay Guan Yeoh, Ming Teh, Jimmy Bok Yan So, Zhiwei Huang

Proceedings Volume 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry; 89390M (2014) https://doi.org/10.1117/12.2039552
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Raman spectroscopy represents a unique optical vibrational technique based on the fundamental premise of inelastic light scattering. Raman spectroscopy enables histopathological assessment at the biomolecular level. We have developed a fiber-optic depth-resolved near-infrared (NIR) Raman endoscopy technique integrated with on-line diagnostic algorithms for in vivo real-time epithelial diagnostics under multimodal wide-field imaging (i.e., white light reflectance (WLR), narrow-band imaging (NBI), autofluorescence imaging (AFI)) modalities. A selection of 450 patients who previously underwent Raman endoscopy (n=1900 spectra) was used to render diagnostic models for identifying gastric precancer (i.e., dysplasia) based on probabilistic partial least squares (PLS) - discriminant analysis (DA). The on-line Raman endoscopy technique was tested prospectively on (n=5) patients for real-time in vivo gastric epithelium tissue diagnosis. The fiber-optic confocal Raman endoscopic technique developed could prospectively identify gastric dysplasia in real-time with a sensitivity: 81.3% (61/75) and specificity 88.3% (188/213) on spectrum basis. On lesion basis, all dysplastic lesions were identified. This study successfully demonstrates for the first time the prospective real-time in vivo diagnosis of gastric precancer using depth-resolved Raman endoscopy.

Citation Download Citation

Mads Sylvest Bergholt, Wei Zheng, Khek Yu Ho, Khay Guan Yeoh, Ming Teh, Jimmy Bok Yan So, and Zhiwei Huang "Real-time depth-resolved fiber optic Raman endoscopy for in vivo diagnosis of gastric precancer", Proc. SPIE 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, 89390M (4 March 2014); https://doi.org/10.1117/12.2039552

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