Two-photon absorption-induced photoacoustic and luminescence imaging employing a femtosecond laser

Gregor Langer; Istvan Attila Veres; Klaus-Dieter Bouchal; Roland Galos; Jakob Kilgus; Peter Burgholzer; Thomas Berer

doi:10.1117/12.2037313

3 March 2014 Two-photon absorption-induced photoacoustic and luminescence imaging employing a femtosecond laser

Gregor Langer, Istvan Attila Veres, Klaus-Dieter Bouchal, Roland Galos, Jakob Kilgus, Peter Burgholzer, Thomas Berer

Proceedings Volume 8943, Photons Plus Ultrasound: Imaging and Sensing 2014; 89431V (2014) https://doi.org/10.1117/12.2037313
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

We present two-photon absorption-induced photoacoustic measurements. Since two-photon photoacoustics is a nonlinear effect numerous physical effects might lead to similar observations. In the presented paper we focus on the effect of the temperature dependent Grüneisen coefficient of water. We discuss the expected theoretical behavior and compare measurements with theoretical predictions. Finally, we demonstrate multimodal photoacoustic and luminescence microscopy via two-photon absorption, and show that it is possible to image objects with localized dyes. Excitation is performed using a femtosecond laser with a wavelength of 800nm. The photoacoustic signals, the luminescence intensity and its spectrum were detected simultaneously.

Citation Download Citation

Gregor Langer, Istvan Attila Veres, Klaus-Dieter Bouchal, Roland Galos, Jakob Kilgus, Peter Burgholzer, and Thomas Berer "Two-photon absorption-induced photoacoustic and luminescence imaging employing a femtosecond laser", Proc. SPIE 8943, Photons Plus Ultrasound: Imaging and Sensing 2014, 89431V (3 March 2014); https://doi.org/10.1117/12.2037313

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