Dental pulp stem cells (DPSCs) differentiation study by confocal Raman microscopy

H. Salehi; P.-Y. Collart-Dutilleul; C. Gergely; F. J. G. Cuisinier

doi:10.1117/12.2041346

4 March 2014 Dental pulp stem cells (DPSCs) differentiation study by confocal Raman microscopy

H. Salehi, P.-Y. Collart-Dutilleul, C. Gergely, F. J. G. Cuisinier

Proceedings Volume 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII; 89470O (2014) https://doi.org/10.1117/12.2041346
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Regenerative medicine brings a huge application for Mesenchymal stem cells such as Dental Pulp Stem Cells (DPSCs). Confocal Raman microscopy, a non-invasive, label free , real time and high spatial resolution imaging technique is used to study osteogenic differentiation of DPSCs. Integrated Raman intensities in the 2800-3000 cm^-1 region (C-H stretching) and 960 cm^-1 peak (phosphate PO₄ ^3-) were collected. In Dental Pulp Stem Cells 21^st day differentiated in buffer solution, phosphate peaks ν₁ PO₄ ^3- (first vibrational mode) at 960cm^-1 and ν₂ PO₄ ^3- at 430cm^–1 and ν₄ PO₄ ^3- at 585cm^-1 are obviously present. Confocal Raman microscopy enables the detection of cell differentiation and it can be used to investigate clinical stem cell research.

Citation Download Citation

H. Salehi, P.-Y. Collart-Dutilleul, C. Gergely, and F. J. G. Cuisinier "Dental pulp stem cells (DPSCs) differentiation study by confocal Raman microscopy", Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89470O (4 March 2014); https://doi.org/10.1117/12.2041346

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KEYWORDS

Raman spectroscopy

Confocal microscopy

Stem cells

Microscopy

Tissues

Data analysis

Proteins

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