Multiphoton microscopy using frequency-doubled compact femtosecond erbium-doped fiber laser

Lin Huang; Shau Poh Chong; Arthur Mills; David Jones; Shuo Tang

doi:10.1117/12.2036052

28 February 2014 Multiphoton microscopy using frequency-doubled compact femtosecond erbium-doped fiber laser

Lin Huang, Shau Poh Chong, Arthur Mills, David Jones, Shuo Tang

Proceedings Volume 8948, Multiphoton Microscopy in the Biomedical Sciences XIV; 894826 (2014) https://doi.org/10.1117/12.2036052
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

We report on the development of a compact multiphoton microscopy (MPM) system based on a frequency-doubled, femtosecond erbium-doped fiber laser source at 1.58 μm. By use of periodically poled MgO:LiNbO₃, frequency-doubled pulses at 790 nm with average power of 75 mW and pulse width of 130 fs are applied as the excitation source. The fiber laser is optimized for its parameters along with the dispersive properties of the delivery fiber such that the MPM signal is maximized at the sample location. Micro-electro-mechanical system (MEMS) scanner, miniature objective, and multimode fiber are further used to make the MPM system compact. MPM images are obtained from unstained biological samples. The MPM system with a compact, portable, low-cost fiber laser has a great potential to transform the bench-top MPM system to a portable system for in vivo MPM imaging.

Citation Download Citation

Lin Huang, Shau Poh Chong, Arthur Mills, David Jones, and Shuo Tang "Multiphoton microscopy using frequency-doubled compact femtosecond erbium-doped fiber laser ", Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 894826 (28 February 2014); https://doi.org/10.1117/12.2036052

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