Correction of image artifacts caused by refractive index gradients in scanning laser optical tomography

Georgios Christian Antonopoulos; Dimitri Pscheniza; Raoul-Amadeus Lorbeer; Marko Heidrich; Kristin Schwanke; Robert Zweigerdt; Tammo Ripken; Heiko Meyer

doi:10.1117/12.2037581

12 March 2014 Correction of image artifacts caused by refractive index gradients in scanning laser optical tomography

Georgios Christian Antonopoulos, Dimitri Pscheniza, Raoul-Amadeus Lorbeer, Marko Heidrich, Kristin Schwanke, Robert Zweigerdt, Tammo Ripken, Heiko Meyer

Author Affiliations +

Proceedings Volume 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI; 894907 (2014) https://doi.org/10.1117/12.2037581
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

We present a technique for correcting image artifacts caused by refractive index distributions in Scanning Laser Optical Tomography (SLOT) and Optical Projection Tomography (OPT). Projection images can be distorted due to the presence of a refractive index distribution around the sample. We consider the special case of a refractive index distribution given by a capillary around a sample. The particular application we are interested in is in vitro imaging of cell spheroids in a glass capillary. Numerical simulations and experimental results are used to illustrate the connection between the Radon transform and the refracted projection. Thereupon we will describe a technique that transforms refracted projections to parallel ray Radon projections and thus allows artifact free reconstruction within the sample volume.

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Georgios Christian Antonopoulos, Dimitri Pscheniza, Raoul-Amadeus Lorbeer, Marko Heidrich, Kristin Schwanke, Robert Zweigerdt, Tammo Ripken, and Heiko Meyer "Correction of image artifacts caused by refractive index gradients in scanning laser optical tomography", Proc. SPIE 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI, 894907 (12 March 2014); https://doi.org/10.1117/12.2037581

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