Single-molecule fluorescence inside solid-state nanochannels

Siddharth Ghosh; Manoj Kumbhakar; Mitja Platen; Ingo Gregor; Jörg Enderlein

doi:10.1117/12.2035236

4 March 2014 Single-molecule fluorescence inside solid-state nanochannels

Siddharth Ghosh, Manoj Kumbhakar, Mitja Platen, Ingo Gregor, Jörg Enderlein

Proceedings Volume 8950, Single Molecule Spectroscopy and Superresolution Imaging VII; 895008 (2014) https://doi.org/10.1117/12.2035236
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

One major limitation of single-molecule fluorescence spectroscopy is the finite residence time of diffusing molecules in the confocal detection volume. The time ranges in the order of milliseconds. This has typical size of femtoliter. Here, we present a concept of extending the residence time using nanochannels of ca. 60 nm x 60 nm cross-section to restrict the molecular motion. We use solid-state nanochannels of silicon and silicon dioxide. This work is aimed to use for dual-focus fluorescence detection combining Anti-Brownian ELectrophoretic or ABEL trap to actively trap single molecule.

Citation Download Citation

Siddharth Ghosh, Manoj Kumbhakar, Mitja Platen, Ingo Gregor, and Jörg Enderlein "Single-molecule fluorescence inside solid-state nanochannels", Proc. SPIE 8950, Single Molecule Spectroscopy and Superresolution Imaging VII, 895008 (4 March 2014); https://doi.org/10.1117/12.2035236

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