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12 March 2014 Fluorescent imaging over an ultra-large field-of-view of 532 cm2 using a flatbed scanner
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We introduce a fluorescent imaging method that is capable of detecting fluorescent micro-particles over an ultra-wide field of view of 19 cm × 28 cm using a modified flatbed scanner. We added a custom-designed absorbing emission filter, a computer controlled two dimensional LED array, and modified the driver of the scanner to maximize the sensitivity, exposure time, and gain for fluorescent detection of micro-objects. This high-throughput fluorescent imaging device used in conjunction with a microfluidic sample holder enables rapid screening of fluorescent micro-objects inside more than 2.2mL of optically dense media (i.e., whole blood) within 5 minutes. The device is sensitive enough to detect fluorescently labeled cells, and generates images that have an effective pixel count of 2.2 Giga-pixels.
© (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Zoltán S. Göröcs, Yuye Ling, Meng D. Yu, Dimitri Karahalios, Kian Mogharabi, Kenny Lu, Qingshan Wei, and Aydogan Ozcan "Fluorescent imaging over an ultra-large field-of-view of 532 cm2 using a flatbed scanner", Proc. SPIE 8951, Optical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics, 89510D (12 March 2014);

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