Photoirradiation study of gold nanospheres and rods in Vero and Hela cell lines

Poorani Gananathan; Prakasarao Aruna; Singaravelu Ganesan; Manickan Elanchezhiyan

doi:10.1117/12.2041933

24 March 2014 Photoirradiation study of gold nanospheres and rods in Vero and Hela cell lines

Poorani Gananathan, Prakasarao Aruna, Singaravelu Ganesan, Manickan Elanchezhiyan

Proceedings Volume 8955, Colloidal Nanoparticles for Biomedical Applications IX; 89551T (2014) https://doi.org/10.1117/12.2041933
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Photoirradiation effect of gold nanospheres in conjucation with green light and rods in conjugation with red light corresponds to their absorption wavelength range found to be appreciable. In this present work concentration of nanomaterial and light dose were optimized. Gold nanospheres were synthesized by reduction technique using Sodium Borohydrate as reducing agent and Trisodium Citrate as capping agent. Au nanorods having 680-900nm absorption were synthesized using reduction techniques with CTAB and BDAC polymers. From UV-Vis absorption and Transmission Electron Microscopy the size of nanoparticles were confirmed. 30nm Gold nanospheres and green light source of 530nm wavelength with power 30mW were applied to Vero and Hela cell lines shows higher toxicity for Hela cells. Nanorods were applied and irradiated with 680nm wavelength light source with light intensity 45mW. Post irradiation effect for 24hrs, 48hrs confirms cell proliferation in normal rate in viable cells. The morphological changes in irradiated spot leads to apoptotoic cell death was confirmed with microscopic imaging. The LD50 value was also calculated.

Citation Download Citation

Poorani Gananathan, Prakasarao Aruna, Singaravelu Ganesan, and Manickan Elanchezhiyan "Photoirradiation study of gold nanospheres and rods in Vero and Hela cell lines", Proc. SPIE 8955, Colloidal Nanoparticles for Biomedical Applications IX, 89551T (24 March 2014); https://doi.org/10.1117/12.2041933

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