Confinement of single macromolecules in free solution using a hydrodynamic trap

Melikhan Tanyeri

doi:10.1117/12.2039063

6 March 2014 Confinement of single macromolecules in free solution using a hydrodynamic trap

Melikhan Tanyeri

Proceedings Volume 8976, Microfluidics, BioMEMS, and Medical Microsystems XII; 89760F (2014) https://doi.org/10.1117/12.2039063
Event: SPIE MOEMS-MEMS, 2014, San Francisco, California, United States

Abstract

We demonstrate the feasibility of trapping and manipulating individual macromolecules such as globular proteins (~ 5 nm in diameter) in free solution using a flow-based, microfluidic confinement method. This new method enables confinement of small nanoscale objects in free solution by utilizing a planar extensional flow created at a microchannel junction. The fluid flow based confinement method described in this work expands the micro/nanomanipulation toolbox by offering a powerful and versatile platform for non-perturbative observation and analysis of single macromolecules in free solution without force fields (electrical, magnetic, optical and acoustic) and surface immobilization.

Citation Download Citation

Melikhan Tanyeri "Confinement of single macromolecules in free solution using a hydrodynamic trap", Proc. SPIE 8976, Microfluidics, BioMEMS, and Medical Microsystems XII, 89760F (6 March 2014); https://doi.org/10.1117/12.2039063

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