Parallel optical sorting of biological cells using the generalized phase contrast method

Lars Rindorf; Minqiang Bu; Jesper Glückstad

doi:10.1117/12.2035321

6 March 2014 Parallel optical sorting of biological cells using the generalized phase contrast method

Lars Rindorf, Minqiang Bu, Jesper Glückstad

Proceedings Volume 8976, Microfluidics, BioMEMS, and Medical Microsystems XII; 89760U (2014) https://doi.org/10.1117/12.2035321
Event: SPIE MOEMS-MEMS, 2014, San Francisco, California, United States

Abstract

Optical forces are used to fixate biological cells with optical tweezers where numerous biological parameters and phenomena can be studied. Optical beams carry a small momentum which generates a weak optical force, but on a cellular level this force is strong enough to allow for manipulation of biological cells in microfluidic systems exclusively using light. We demonstrate an optical cell sorter that uses simultaneous manipulation by multiple laser beams using the Generalized Phase Contrast method (GPC). The basic principle in an optical sorter is that the radiation force of the optical beam can push the biological cell from one microfluidic sheath flow to another. By incorporating a spatial light modulator the manipulation can be made parallel with multiple laser beams. We claim advantages over the serial optical sorters with only a single laser beam that has been demonstrated by others.

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Lars Rindorf, Minqiang Bu, and Jesper Glückstad "Parallel optical sorting of biological cells using the generalized phase contrast method ", Proc. SPIE 8976, Microfluidics, BioMEMS, and Medical Microsystems XII, 89760U (6 March 2014); https://doi.org/10.1117/12.2035321

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KEYWORDS

Microfluidics

Spatial light modulators

Light scattering

Biomedical optics

Refractive index

Scattering

Particles

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