Label-free electronic detection of target cells

Rahim Esfandyarpour; Mehdi Javanmard; James Harris; Ronald W. Davis

doi:10.1117/12.2037966

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6 March 2014 Label-free electronic detection of target cells

Rahim Esfandyarpour, Mehdi Javanmard, James Harris, Ronald W. Davis

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Proceedings Volume 8976, Microfluidics, BioMEMS, and Medical Microsystems XII; 897618 (2014) https://doi.org/10.1117/12.2037966
Event: SPIE MOEMS-MEMS, 2014, San Francisco, California, United States

Abstract

In this manuscript we describe an electronic label-free method for detection of target cells, which has potential applications ranging from pathogen detection for food safety all the way to detection of circulating tumor cells for cancer diagnosis. The nanoelectronic platform consists of a stack of electrodes separated by a 30nm thick insulating layer. Cells binding to the tip of the sensor result in a decrease in the impedance at the sensing tip due to an increase in the fringing capacitance between the electrodes. As a proof of concept we demonstrate the ability to detect Saccharomyces Cerevisae cells with high specificity using a sensor functionalized with Concanavalin A. Ultimately we envision using this sensor in conjunction with a technology for pre-concentration of target cells to develop a fully integrated micro total analysis system.

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Rahim Esfandyarpour, Mehdi Javanmard, James Harris, and Ronald W. Davis "Label-free electronic detection of target cells", Proc. SPIE 8976, Microfluidics, BioMEMS, and Medical Microsystems XII, 897618 (6 March 2014); https://doi.org/10.1117/12.2037966

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