3D shape analysis of heterochromatin foci based on a 3D spherical harmonics intensity model

Simon Eck; Stefan Wörz; Katharina Müller-Ott; Matthias Hahn; Gunnar Schotta; Karsten Rippe; Karl Rohr

doi:10.1117/12.2043482

21 March 2014 3D shape analysis of heterochromatin foci based on a 3D spherical harmonics intensity model

Simon Eck, Stefan Wörz, Katharina Müller-Ott, Matthias Hahn, Gunnar Schotta, Karsten Rippe, Karl Rohr

Proceedings Volume 9034, Medical Imaging 2014: Image Processing; 90340X (2014) https://doi.org/10.1117/12.2043482
Event: SPIE Medical Imaging, 2014, San Diego, California, United States

Abstract

We propose a novel approach for 3D shape analysis of heterochromatin foci in 3D confocal light microscopy images of cell nuclei. The approach is based on a 3D parametric intensity model and uses a spherical harmonics (SH) expansion. The model parameters including the SH coefficients are automatically determined by least squares fitting of the model to the image intensities. Based on the obtained SH coefficients, a shape descriptor is determined, which enables distinguishing heterochromatin foci based on their 3D shape to characterize compaction states of heterochromatin. Our approach has been successfully applied to real static and dynamic 3D microscopy image data.

Citation Download Citation

Simon Eck, Stefan Wörz, Katharina Müller-Ott, Matthias Hahn, Gunnar Schotta, Karsten Rippe, and Karl Rohr "3D shape analysis of heterochromatin foci based on a 3D spherical harmonics intensity model", Proc. SPIE 9034, Medical Imaging 2014: Image Processing, 90340X (21 March 2014); https://doi.org/10.1117/12.2043482

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