A microfluidic platform with integrated arrays for immunologic assays for biological pathogen detection

Richard Klemm; Holger Becker; Nadine Hlawatsch; Sandra Julich; Peter Miethe; Christian Moche; Sebastian Schattschneider; Herbert Tomaso; Claudia Gärtner

doi:10.1117/12.2050269

29 May 2014 A microfluidic platform with integrated arrays for immunologic assays for biological pathogen detection

Richard Klemm, Holger Becker, Nadine Hlawatsch, Sandra Julich, Peter Miethe, Christian Moche, Sebastian Schattschneider, Herbert Tomaso, Claudia Gärtner

Author Affiliations +

Proceedings Volume 9073, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XV; 907311 (2014) https://doi.org/10.1117/12.2050269
Event: SPIE Defense + Security, 2014, Baltimore, MD, United States

Abstract

The ability to integrate complete assays on a microfluidic chip helps to greatly simplify instrument requirements and allows the use of lab-on-a-chip technology in the field. A core application for such field-portable systems is the detection of pathogens in a CBRN scenario such as permanent monitoring of airborne pathogens, e.g. in subway stations or hospitals etc. An immunological assay was chosen as method for the pathogen identification. The conceptual approach was its realization as a lab-on-a-chip system, enabling an easy handling of the sample in an automated manner. The immunological detection takes place on an antibody array directly implemented in the microfluidic network. Different immobilization strategies will be presented showing the performance of the system. Central elements of the disposable microfluidic device like fluidic interface, turning valves, liquid introduction and waste storage, as well as the architecture of measurement and control fluidic network, will be introduced. Overall process times of about 30 minutes were achieved and assays for the detection of Francisella tularensis and Yersinia pestis are presented. An important feature of the integrated lab-on-a-chip approach is that all waste liquids remain on-chip and contamination risks can be avoided.

Citation Download Citation

Richard Klemm, Holger Becker, Nadine Hlawatsch, Sandra Julich, Peter Miethe, Christian Moche, Sebastian Schattschneider, Herbert Tomaso, and Claudia Gärtner "A microfluidic platform with integrated arrays for immunologic assays for biological pathogen detection", Proc. SPIE 9073, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XV, 907311 (29 May 2014); https://doi.org/10.1117/12.2050269

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available