Conformational Distributions Of Flexible Molecules From Steady State Fluorescence Energy Transfer

Wieslaw Wiczk; Ignacy Grvczvnski; Michael L Johnson; Joseph R Lakowicz

doi:10.1117/12.945401

24 June 1988 Conformational Distributions Of Flexible Molecules From Steady State Fluorescence Energy Transfer

Wieslaw Wiczk, Ignacy Grvczvnski, Michael L Johnson, Joseph R Lakowicz

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945401
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

We demonstrate that distance distributions between two sites on flexible molecules can be recovered using steady state measurements of fluorescence energy transfer. The characteristic Forster distance (R_°) for energy transfer was varied by collisional quenching of the donor, which decreases its quantum yield. The measured transfer efficiencies for each value of R. provide a different average of the distance distribution. The R.-dependent transfer efficiencies, when analyzed by non linear least- squares, were found to reliably determine the distance distribution, as shown by agreement with the frequency-domain method (Chem. Phys. Lett. 1987, 138:587-593).

Citation Download Citation

Wieslaw Wiczk, Ignacy Grvczvnski, Michael L Johnson, and Joseph R Lakowicz "Conformational Distributions Of Flexible Molecules From Steady State Fluorescence Energy Transfer", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945401

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