Fluorescence Spectroscopy Data Analysis Environment: A Second Generation Global Analysis Program

Joseph M Beechem; Enrico Gratton

doi:10.1117/12.945370

24 June 1988 Fluorescence Spectroscopy Data Analysis Environment: A Second Generation Global Analysis Program

Joseph M Beechem, Enrico Gratton

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945370
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

A fluorescence spectroscopy global analysis environment is described. Within this analysis environment, multidimensional fluorescence decay data (time and frequency domains) can be analyzed in terms of a wide variety of photophysical models. A generalized compartmental analysis structure is utilized, where one can specify the functions used to link the various compartments together. All fitting parameters may be characterized by either discrete or distributed values. Multi-temperature global analysis of frequency domain fluorescence studies from a single tryptophan containing protein are described.

Citation Download Citation

Joseph M Beechem and Enrico Gratton "Fluorescence Spectroscopy Data Analysis Environment: A Second Generation Global Analysis Program", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945370

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