Zinc oxide nanostructures for electrochemical cortisol biosensing

Phani Kiran Vabbina; Ajeet Kaushik; Kathryn Tracy; Shekhar Bhansali; Nezih Pala

doi:10.1117/12.2051156

22 May 2014 Zinc oxide nanostructures for electrochemical cortisol biosensing

Phani Kiran Vabbina, Ajeet Kaushik, Kathryn Tracy, Shekhar Bhansali, Nezih Pala

Proceedings Volume 9107, Smart Biomedical and Physiological Sensor Technology XI; 91070U (2014) https://doi.org/10.1117/12.2051156
Event: SPIE Sensing Technology + Applications, 2014, Baltimore, MD, United States

Abstract

In this paper, we report on fabrication of a label free, highly sensitive and selective electrochemical cortisol immunosensors using one dimensional (1D) ZnO nanorods (ZnO-NRs) and two dimensional nanoflakes (ZnO-NFs) as immobilizing matrix. The synthesized ZnO nanostructures (NSs) were characterized using scanning electron microscopy (SEM), selective area diffraction (SAED) and photoluminescence spectra (PL) which showed that both ZnO-NRs and ZnO-NFs are single crystalline and oriented in [0001] direction. Anti-cortisol antibody (Anti-C_ab) are used as primary capture antibodies to detect cortisol using electrochemical impedance spectroscopy (EIS). The charge transfer resistance increases linearly with increase in cortisol concentration and exhibits a sensitivity of 3.078 KΩ. M^-1 for ZnO-NRs and 540 Ω. M ^-1 for ZnO-NFs. The developed ZnO-NSs based immunosensor is capable of detecting cortisol at 1 pM. The observed sensing parameters are in physiological range. The developed sensors can be integrated with microfluidic system and miniaturized potentiostat to detect cortisol at point-of-care.

Citation Download Citation

Phani Kiran Vabbina, Ajeet Kaushik, Kathryn Tracy, Shekhar Bhansali, and Nezih Pala "Zinc oxide nanostructures for electrochemical cortisol biosensing", Proc. SPIE 9107, Smart Biomedical and Physiological Sensor Technology XI, 91070U (22 May 2014); https://doi.org/10.1117/12.2051156

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