Peptide-based protein capture agents with high affinity, selectivity, and stability as antibody replacements in biodetection assays

Matthew B. Coppock; Blake Farrow; Candice Warner; Amethist S. Finch; Bert Lai; Deborah A. Sarkes; James R. Heath; Dimitra Stratis-Cullum

doi:10.1117/12.2052542

22 May 2014 Peptide-based protein capture agents with high affinity, selectivity, and stability as antibody replacements in biodetection assays

Matthew B. Coppock, Blake Farrow, Candice Warner, Amethist S. Finch, Bert Lai, Deborah A. Sarkes, James R. Heath, Dimitra Stratis-Cullum

Author Affiliations +

Proceedings Volume 9107, Smart Biomedical and Physiological Sensor Technology XI; 910711 (2014) https://doi.org/10.1117/12.2052542
Event: SPIE Sensing Technology + Applications, 2014, Baltimore, MD, United States

Abstract

Current biodetection assays that employ monoclonal antibodies as primary capture agents exhibit limited fieldability, shelf life, and performance due to batch-to-batch production variability and restricted thermal stability. In order to improve upon the detection of biological threats in fieldable assays and systems for the Army, we are investigating protein catalyzed capture (PCC) agents as drop-in replacements for the existing antibody technology through iterative in situ click chemistry. The PCC agent oligopeptides are developed against known protein epitopes and can be mass produced using robotic methods. In this work, a PCC agent under development will be discussed. The performance, including affinity, selectivity, and stability of the capture agent technology, is analyzed by immunoprecipitation, western blotting, and ELISA experiments. The oligopeptide demonstrates superb selectivity coupled with high affinity through multi-ligand design, and improved thermal, chemical, and biochemical stability due to non-natural amino acid PCC agent design.

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Matthew B. Coppock, Blake Farrow, Candice Warner, Amethist S. Finch, Bert Lai, Deborah A. Sarkes, James R. Heath, and Dimitra Stratis-Cullum "Peptide-based protein capture agents with high affinity, selectivity, and stability as antibody replacements in biodetection assays", Proc. SPIE 9107, Smart Biomedical and Physiological Sensor Technology XI, 910711 (22 May 2014); https://doi.org/10.1117/12.2052542

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