Bacteriophage adhesin-coated long-period gratings for bacterial lipopolysaccharide recognition

Marcin Koba; Mateusz Śmietana; Ewa Brzozowska; Sabina Górska; Predrag Mikulic; Wojtek J. Bock

doi:10.1117/12.2059618

2 June 2014 Bacteriophage adhesin-coated long-period gratings for bacterial lipopolysaccharide recognition

Marcin Koba, Mateusz Śmietana, Ewa Brzozowska, Sabina Górska, Predrag Mikulic, Wojtek J. Bock

Proceedings Volume 9157, 23rd International Conference on Optical Fibre Sensors; 915757 (2014) https://doi.org/10.1117/12.2059618
Event: OFS2014 23rd International Conference on Optical Fiber Sensors, 2014, Santander, Spain

Abstract

In this work we report an application of the optical fiber long-period gratings (LPGs) working near the dispersion turning point of higher order cladding modes for bacterial lipopolysaccharide (LPS) recognition. We show that when the LPG is functionalized with the bacteriophage adhesin, it is capable of very specific LPS detection. Thus, we compare label-free binding effect for specific to the adhesin LPS-positive and non-specific LPS-negative. The resonance wavelength shift induced by the LPS-positive reaches 2.9 nm, while for LPS-negative the shift is negligible. The LPG-based sensing structure allows for monitoring of the binding phenomenon in real time and with good accuracy.

Citation Download Citation

Marcin Koba, Mateusz Śmietana, Ewa Brzozowska, Sabina Górska, Predrag Mikulic, and Wojtek J. Bock "Bacteriophage adhesin-coated long-period gratings for bacterial lipopolysaccharide recognition", Proc. SPIE 9157, 23rd International Conference on Optical Fibre Sensors, 915757 (2 June 2014); https://doi.org/10.1117/12.2059618

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