The Pocketscope: a spatial light modulator based epi-fluorescence microscope for optogenetics

Anna Linnenberger; Darcy S. Peterka; Sean Quirin; Rafael Yuste

doi:10.1117/12.2064304

16 September 2014 The Pocketscope: a spatial light modulator based epi-fluorescence microscope for optogenetics

Anna Linnenberger, Darcy S. Peterka, Sean Quirin, Rafael Yuste

Proceedings Volume 9164, Optical Trapping and Optical Micromanipulation XI; 91640Y (2014) https://doi.org/10.1117/12.2064304
Event: SPIE NanoScience + Engineering, 2014, San Diego, California, United States

Abstract

Microscopy incorporating spatial light modulators (SLMs) enables three dimensional (3D) excitation and monitoring of the activity of neuronal ensembles, enabling studies of neuronal circuit activity both in vitro and in vivo. In this paper we present a portable (22 cm x 42.5 cm x 30 cm), SLM-based epi-fluorescence upright microscope (“Pocketscope”) that enables 3D calcium imaging and photoactivation of neurons in brain slices. Here we describe the implementation of the instrument; quantify the volume over which neural activity can be excited; and demonstrate the use of the system for mapping neural circuits in brain slices.

Citation Download Citation

Anna Linnenberger, Darcy S. Peterka, Sean Quirin, and Rafael Yuste "The Pocketscope: a spatial light modulator based epi-fluorescence microscope for optogenetics", Proc. SPIE 9164, Optical Trapping and Optical Micromanipulation XI, 91640Y (16 September 2014); https://doi.org/10.1117/12.2064304

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