Novel pH-sensitive probes with a ratiometric detection for intracellular pH

Martin Ipuy; Cyrielle Billon; Guillaume Micouin; Jacques Samarut; Chantal Andraud; Yann Bretonnière

doi:10.1117/12.2066132

27 August 2014 Novel pH-sensitive probes with a ratiometric detection for intracellular pH

Martin Ipuy, Cyrielle Billon, Guillaume Micouin, Jacques Samarut, Chantal Andraud, Yann Bretonnière

Proceedings Volume 9171, Nanobiosystems: Processing, Characterization, and Applications VII; 91710Q (2014) https://doi.org/10.1117/12.2066132
Event: SPIE NanoScience + Engineering, 2014, San Diego, California, United States

Abstract

The development of new pH-sensitive fluorescent probes based on a push-pull architecture is presented with a 2- dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofurane as strong electron acceptor group. With a small structural change, it is possible to obtain a large range of phenolic pKa from 4.8 to 8.6 with some close to neutrality, underlining the role of the electron density modulation on the acidic properties. Remarkable changes in the optical properties (both absorption and fluorescence) were observed as a function of the pH. Ratiometric imaging of intracellular pH was carried out with the most promising probes and highlighted the possibility to distinguish near-neutral minor pH fluctuations in cells.

Citation Download Citation

Martin Ipuy, Cyrielle Billon, Guillaume Micouin, Jacques Samarut, Chantal Andraud, and Yann Bretonnière "Novel pH-sensitive probes with a ratiometric detection for intracellular pH", Proc. SPIE 9171, Nanobiosystems: Processing, Characterization, and Applications VII, 91710Q (27 August 2014); https://doi.org/10.1117/12.2066132

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