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16 September 2014A novel approach for scanning electron microscopic observation in atmospheric pressure
Atmospheric scanning electron microscopy (ASEM) for observing samples at ambient atmospheric pressure is
introduced in this study. An additional specimen chamber with a thin membrane allowing electron beam propagation is
inserted in the main specimen chamber. Close proximity of the sample to the membrane enables the detection of backscattered
electrons (BSEs) sufficient for imaging. A probability analysis of the un-scattered fraction of the incident
electron beam and the beam profile further supports the feasibility of atmospheric SEM imaging over a controlled
membrane-sample distance. An image enhancement method based on the analysis is introduced for the ASEM.