Quantitative interferometric microscopic flow cytometer with expanded principal component analysis method

Shouyu Wang; Ying Jin; Keding Yan; Liang Xue; Fei Liu; Zhenhua Li

doi:10.1117/12.2070970

20 November 2014 Quantitative interferometric microscopic flow cytometer with expanded principal component analysis method

Shouyu Wang, Ying Jin, Keding Yan, Liang Xue, Fei Liu, Zhenhua Li

Author Affiliations +

Proceedings Volume 9268, Optics in Health Care and Biomedical Optics VI; 92681G (2014) https://doi.org/10.1117/12.2070970
Event: SPIE/COS Photonics Asia, 2014, Beijing, China

Abstract

Quantitative interferometric microscopy is used in biological and medical fields and a wealth of applications are proposed in order to detect different kinds of biological samples. Here, we develop a phase detecting cytometer based on quantitative interferometric microscopy with expanded principal component analysis phase retrieval method to obtain phase distributions of red blood cells with a spatial resolution ~1.5 μm. Since expanded principal component analysis method is a time-domain phase retrieval algorithm, it could avoid disadvantages of traditional frequency-domain algorithms. Additionally, the phase retrieval method realizes high-speed phase imaging from multiple microscopic interferograms captured by CCD camera when the biological cells are scanned in the field of view. We believe this method can be a powerful tool to quantitatively measure the phase distributions of different biological samples in biological and medical fields.

Citation Download Citation

Shouyu Wang, Ying Jin, Keding Yan, Liang Xue, Fei Liu, and Zhenhua Li "Quantitative interferometric microscopic flow cytometer with expanded principal component analysis method", Proc. SPIE 9268, Optics in Health Care and Biomedical Optics VI, 92681G (20 November 2014); https://doi.org/10.1117/12.2070970

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