Monitoring the effect of low-level laser therapy in healing process of skin with second harmonic generation imaging techniques

Xiaoman Zhang; Biying Yu; Cuncheng Weng; Hui Li

doi:10.1117/12.2071927

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20 November 2014 Monitoring the effect of low-level laser therapy in healing process of skin with second harmonic generation imaging techniques

Xiaoman Zhang, Biying Yu, Cuncheng Weng, Hui Li

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Proceedings Volume 9268, Optics in Health Care and Biomedical Optics VI; 92682E (2014) https://doi.org/10.1117/12.2071927
Event: SPIE/COS Photonics Asia, 2014, Beijing, China

Abstract

The 632nm wavelength low intensity He-Ne laser was used to irradiated on 15 mice which had skin wound. The dynamic changes and wound healing processes were observed with nonlinear spectral imaging technology. We observed that:(1)The wound healing process was accelerated by the low-level laser therapy(LLLT);(2)The new tissues produced second harmonic generation (SHG) signals. Collagen content and microstructure differed dramatically at different time pointed along the wound healing. Our observation shows that the low intensity He-Ne laser irradiation can accelerate the healing process of skin wound in mice, and SHG imaging technique can be used to observe wound healing process, which is useful for quantitative characterization of wound status during wound healing process.

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Xiaoman Zhang, Biying Yu, Cuncheng Weng, and Hui Li "Monitoring the effect of low-level laser therapy in healing process of skin with second harmonic generation imaging techniques", Proc. SPIE 9268, Optics in Health Care and Biomedical Optics VI, 92682E (20 November 2014); https://doi.org/10.1117/12.2071927

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