Ex-vivo multi-modal microscopy of healthy skin

Edgar Guevara; José Manuel Gutiérrez-Hernández; Alexandre Castonguay; Frédéric Lesage; Francisco Javier González

doi:10.1117/12.2073702

25 September 2014 Ex-vivo multi-modal microscopy of healthy skin

Edgar Guevara, José Manuel Gutiérrez-Hernández, Alexandre Castonguay, Frédéric Lesage, Francisco Javier González

Proceedings Volume 9288, Photonics North 2014; 92880P (2014) https://doi.org/10.1117/12.2073702
Event: Photonics North 2014, 2014, Montréal, Canada

Abstract

The thorough characterization of skin samples is a critical step in investigating dermatological diseases. The combination of depth-sensitive anatomical imaging with molecular imaging has the potential to provide vast information about the skin. In this proof-of-concept work we present high-resolution mosaic images of skin biopsies using Optical Coherence Tomography (OCT) manually co-registered with standard microscopy, bi-dimensional Raman spectral mapping and fluorescence imaging. A human breast skin sample, embedded in paraffin, was imaged with a swept-source OCT system at 1310 nm. Individual OCT volumes were acquired in fully automated fashion in order to obtain a large field-of-view at high resolution (~10μm). Based on anatomical features, the other three modalities were manually co-registered to the projected OCT volume, using an affine transformation. A drawback is the manual co-registration, which may limit the utility of this method. However, the results indicate that multiple imaging modalities provide complementary information about the sample. This pilot study suggests that multi-modal microscopy may be a valuable tool in the characterization of skin biopsies.

Citation Download Citation

Edgar Guevara, José Manuel Gutiérrez-Hernández, Alexandre Castonguay, Frédéric Lesage, and Francisco Javier González "Ex-vivo multi-modal microscopy of healthy skin", Proc. SPIE 9288, Photonics North 2014, 92880P (25 September 2014); https://doi.org/10.1117/12.2073702

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