The method for detecting diffusion ring diameter in Hemagglutinin measuring

Wenbo Jing; Xue Liu; Jin Duan; Xiao-man Wang

doi:10.1117/12.2072438

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24 November 2014 The method for detecting diffusion ring diameter in Hemagglutinin measuring

Wenbo Jing, Xue Liu, Jin Duan, Xiao-man Wang

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Proceedings Volume 9301, International Symposium on Optoelectronic Technology and Application 2014: Image Processing and Pattern Recognition; 930123 (2014) https://doi.org/10.1117/12.2072438
Event: International Symposium on Optoelectronic Technology and Application 2014, 2014, Beijing, China

Abstract

The diffuser ring diameter measurement is the most critical in hemagglutinin Measuring. The traditional methods, such as a vernier caliper or high-definition scanned images are subjective and low for the measurement data reliability. Propose high-resolution diffusion ring image for drop-resolution processing, adaptive Canny operator and local detection method to extract complete and clear diffusion ring boundaries, and finally make use of polynomial interpolation algorithm to make diffusion ring outer boundary pixel coordinates achieve sub-pixel accuracy and the least-squares fitting circle algorithm to calculate the precise center of the circle and the diameter of the diffuser ring. Experimental results show that the method detection time is only 63.61ms, which is a faster speed; diffuser ring diameter estimation error can achieve 0.55 pixel, high stability in experimental data. This method is adapted to the various types of influenza vaccine hemagglutinin content measurements, and has important value in the influenza vaccine quality detection.

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Wenbo Jing, Xue Liu, Jin Duan, and Xiao-man Wang "The method for detecting diffusion ring diameter in Hemagglutinin measuring", Proc. SPIE 9301, International Symposium on Optoelectronic Technology and Application 2014: Image Processing and Pattern Recognition, 930123 (24 November 2014); https://doi.org/10.1117/12.2072438

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