Tumor implantation model for rapid testing of lymphatic dye uptake from paw to node in small animals

Alisha V. DSouza; Jonathan T. Elliott; Jason R. Gunn; Richard J. Barth M.D.; Kimberley S. Samkoe; Kenneth M. Tichauer; Brian W. Pogue

doi:10.1117/12.2081196

4 March 2015 Tumor implantation model for rapid testing of lymphatic dye uptake from paw to node in small animals

Alisha V. DSouza, Jonathan T. Elliott, Jason R. Gunn, Richard J. Barth M.D., Kimberley S. Samkoe, Kenneth M. Tichauer, Brian W. Pogue

Author Affiliations +

Proceedings Volume 9311, Molecular-Guided Surgery: Molecules, Devices, and Applications; 93110D (2015) https://doi.org/10.1117/12.2081196
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Morbidity and complexity involved in lymph node staging via surgical resection and biopsy calls for staging techniques that are less invasive. While visible blue dyes are commonly used in locating sentinel lymph nodes, since they follow tumor-draining lymphatic vessels, they do not provide a metric to evaluate presence of cancer. An area of active research is to use fluorescent dyes to assess tumor burden of sentinel and secondary lymph nodes.

The goal of this work was to successfully deploy and test an intra-nodal cancer-cell injection model to enable planar fluorescence imaging of a clinically relevant blue dye, specifically methylene blue – used in the sentinel lymph node procedure – in normal and tumor-bearing animals, and subsequently segregate tumor-bearing from normal lymph nodes. This direct-injection based tumor model was employed in athymic rats (6 normal, 4 controls, 6 cancer-bearing), where luciferase-expressing breast cancer cells were injected into axillary lymph nodes. Tumor presence in nodes was confirmed by bioluminescence imaging before and after fluorescence imaging. Lymphatic uptake from the injection site (intradermal on forepaw) to lymph node was imaged at approximately 2 frames/minute. Large variability was observed within each cohort.

Citation Download Citation

Alisha V. DSouza, Jonathan T. Elliott, Jason R. Gunn, Richard J. Barth M.D., Kimberley S. Samkoe, Kenneth M. Tichauer, and Brian W. Pogue "Tumor implantation model for rapid testing of lymphatic dye uptake from paw to node in small animals", Proc. SPIE 9311, Molecular-Guided Surgery: Molecules, Devices, and Applications, 93110D (4 March 2015); https://doi.org/10.1117/12.2081196

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available