Automated long-term tracking of freely moving animal and functional brain imaging based on fiber optic microscopy

Jaepyeong Cha; Gyeong Woo Cheon; Jin U. Kang

doi:10.1117/12.2077034

5 March 2015 Automated long-term tracking of freely moving animal and functional brain imaging based on fiber optic microscopy

Jaepyeong Cha, Gyeong Woo Cheon, Jin U. Kang

Author Affiliations +

Proceedings Volume 9317, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XV; 931708 (2015) https://doi.org/10.1117/12.2077034
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

In this study, we demonstrate an automated data acquisition/analysis platform for both long-term motion tracking and functional brain imaging in freely moving mice. Our system utilizes a fiber-bundle based fluorescence microscope for 24 hours imaging of cellular activities within the brain while also monitoring corresponding animal behaviors using a NIR camera. Synchronized software and automation of analysis allow quantification of all animal behaviors and their brain activities over extended periods of time. Our platform can be used for interrogation of the brain activities in different behavioral states and is also well-suited for longitudinal studies of cellular activities in freely moving animals.

Citation Download Citation

Jaepyeong Cha, Gyeong Woo Cheon, and Jin U. Kang "Automated long-term tracking of freely moving animal and functional brain imaging based on fiber optic microscopy", Proc. SPIE 9317, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XV, 931708 (5 March 2015); https://doi.org/10.1117/12.2077034

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