Optical clearing of the mouse brain and light attenuation quantitation

Angela d'Esposito; Daniil Nikitichev; Adrien Desjardins; Simon Walker-Samuel; Mark F. Lythgoe

doi:10.1117/12.2075580

13 March 2015 Optical clearing of the mouse brain and light attenuation quantitation

Angela d'Esposito, Daniil Nikitichev, Adrien Desjardins, Simon Walker-Samuel, Mark F. Lythgoe

Proceedings Volume 9321, Optical Interactions with Tissue and Cells XXVI; 93210R (2015) https://doi.org/10.1117/12.2075580
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Optical clearing allows the reduction of light scattering in biological tissue, enabling 3D morphological information to be obtained deep within tissue using techniques such as optical projection tomography and light sheet microscopy. However, the extent of the clearing is dependent on the technique that is used. There is therefore a need for methods to quantify the quality of the clearing process and thereby to compare clearing techniques. In this study, we developed such a method using a custom spectroscopy system and applied it to compare three techniques that were applied to mouse brain: BABB (Murray’s clear), pBABB (a modification of BABB which includes the use of hydrogen peroxide), and passive CLARITY.

Citation Download Citation

Angela d'Esposito, Daniil Nikitichev, Adrien Desjardins, Simon Walker-Samuel, and Mark F. Lythgoe "Optical clearing of the mouse brain and light attenuation quantitation", Proc. SPIE 9321, Optical Interactions with Tissue and Cells XXVI, 93210R (13 March 2015); https://doi.org/10.1117/12.2075580

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