Photoacoustic imaging of single circulating melanoma cells in vivo

Lidai Wang; Junjie Yao; Ruiying Zhang; Song Xu; Guo Li; Jun Zou; Lihong V. Wang

doi:10.1117/12.2076987

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11 March 2015 Photoacoustic imaging of single circulating melanoma cells in vivo

Lidai Wang, Junjie Yao, Ruiying Zhang, Song Xu, Guo Li, Jun Zou, Lihong V. Wang

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Proceedings Volume 9323, Photons Plus Ultrasound: Imaging and Sensing 2015; 93230A (2015) https://doi.org/10.1117/12.2076987
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Melanoma, one of the most common types of skin cancer, has a high mortality rate, mainly due to a high propensity for tumor metastasis. The presence of circulating tumor cells (CTCs) is a potential predictor for metastasis. Label-free imaging of single circulating melanoma cells in vivo provides rich information on tumor progress. Here we present photoacoustic microscopy of single melanoma cells in living animals. We used a fast-scanning optical-resolution photoacoustic microscope to image the microvasculature in mouse ears. The imaging system has sub-cellular spatial resolution and works in reflection mode. A fast-scanning mirror allows the system to acquire fast volumetric images over a large field of view. A 500-kHz pulsed laser was used to image blood and CTCs. Single circulating melanoma cells were imaged in both capillaries and trunk vessels in living animals. These high-resolution images may be used in early detection of CTCs with potentially high sensitivity. In addition, this technique enables in vivo study of tumor cell extravasation from a primary tumor, which addresses an urgent pre-clinical need.

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Lidai Wang, Junjie Yao, Ruiying Zhang, Song Xu, Guo Li, Jun Zou, and Lihong V. Wang "Photoacoustic imaging of single circulating melanoma cells in vivo", Proc. SPIE 9323, Photons Plus Ultrasound: Imaging and Sensing 2015, 93230A (11 March 2015); https://doi.org/10.1117/12.2076987

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