Software-aided automatic cell optoporation system

Hans Georg Breunig; Ana Batista; Aisada Uchugonova; Karsten König

doi:10.1117/12.2078728

2 March 2015 Software aided automatic cell optoporation system

Hans Georg Breunig, Ana Batista, Aisada Uchugonova, Karsten König

Proceedings Volume 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII; 93280D (2015) https://doi.org/10.1117/12.2078728
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Optoporation, the laser-induced transient cell membrane perforation, has emerged as a powerful non-invasive and highly efficient cell-transfection technique. It is usually done by targeting individual cells manually which significantly limits number of addressable cells. We present an experimental setup with custom-made software control for automated cell optoporation experiments. The automated software evaluates contrast edges in bright-field sample images to identify cell locations for laser illumination and controls the hardware to transiently laser-illuminate these positions. By software controlled stitching together of several microscopic field-of-views in principle all cells in a culture dish can be targeted without further user interaction. The software-based automation allows to significantly increase the number of treatable cells compared to a manual approach. We illustrate the experimental capabilities in CHO-cell optoporation experiments with highly focused beam-shaped sub-15 fs pulses.

Citation Download Citation

Hans Georg Breunig, Ana Batista, Aisada Uchugonova, and Karsten König "Software aided automatic cell optoporation system", Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93280D (2 March 2015); https://doi.org/10.1117/12.2078728

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