Multimodal optical phenotyping of cancer cells

Lena Kastl; Björn Budde; Michael Isbach; Christina Rommel; Björn Kemper; Jürgen Schnekenburger

doi:10.1117/12.2080151

2 March 2015 Multimodal optical phenotyping of cancer cells

Lena Kastl, Björn Budde, Michael Isbach, Christina Rommel, Björn Kemper, Jürgen Schnekenburger

Proceedings Volume 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII; 93280F (2015) https://doi.org/10.1117/12.2080151
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

There is a growing interest in label-free, optical techniques like digital holographic microscopy (DHM) and optical cell stretching, since the interaction with samples is minimized. Because optical manipulation strongly depends on the optical and physiological properties of the investigated material, we combined the usage of these methods for the characterization of pancreatic tumor cells. Our results demonstrate that cells of distinct differentiation levels, or different expression in only one protein, show differences in their deformability. Additionally, the DHM results showed only few variations in the refractive index, indicating that it does not significantly influence the results of the optical cell stretching. Thus, the combined usage of the two technologies represents a promising new approach for tumor cell characterization.

Citation Download Citation

Lena Kastl, Björn Budde, Michael Isbach, Christina Rommel, Björn Kemper, and Jürgen Schnekenburger "Multimodal optical phenotyping of cancer cells", Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93280F (2 March 2015); https://doi.org/10.1117/12.2080151

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