Two-photon excited fluorescence microendoscopic imaging using a GRIN lens

Wei Yan; Xiao Peng; Danying Lin; Qi Wang; Jian Gao; Jie Zhou; Tong Ye; Junle Qu; Hanben Niu

doi:10.1117/12.2079183

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5 March 2015 Two-photon excited fluorescence microendoscopic imaging using a GRIN lens

Wei Yan, Xiao Peng, Danying Lin, Qi Wang, Jian Gao, Jie Zhou, Tong Ye, Junle Qu, Hanben Niu

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Proceedings Volume 9329, Multiphoton Microscopy in the Biomedical Sciences XV; 93290N (2015) https://doi.org/10.1117/12.2079183
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

With the rapid development of life sciences, there is an increasing demand for intravital fluorescence imaging of small animals. However, large dimensions and limited working distances of objective lenses in traditional fluorescence microscopes have limited the imaging applications mostly to superficial tissues. To overcome this disadvantage, researchers have developed the graded-index (GRIN) probes with small diameters for imaging internal organs of small animals in a minimally invasive fashion. Here, we present the development of a fluorescence endoscopic imaging system based on a GRIN lens using two-photon excitation. Experimental results showed that this system could perform dynamic fluorescence microendoscopic imaging and monitor the blood flow in anesthetized living mice using two-photon excitation.

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Wei Yan, Xiao Peng, Danying Lin, Qi Wang, Jian Gao, Jie Zhou, Tong Ye, Junle Qu, and Hanben Niu "Two-photon excited fluorescence microendoscopic imaging using a GRIN lens", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93290N (5 March 2015); https://doi.org/10.1117/12.2079183

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