Effects of anesthesia on the cerebral capillary blood flow in young and old mice

Mohammad Moeini; Maryam S. Tabatabaei; Samuel Bélanger; Pramod Avti; Alexandre Castonguay; Philippe Pouliot; Frédéric Lesage

doi:10.1117/12.2079803

5 March 2015 Effects of anesthesia on the cerebral capillary blood flow in young and old mice

Mohammad Moeini, Maryam S. Tabatabaei, Samuel Bélanger, Pramod Avti, Alexandre Castonguay, Philippe Pouliot, Frédéric Lesage

Author Affiliations +

Proceedings Volume 9329, Multiphoton Microscopy in the Biomedical Sciences XV; 932929 (2015) https://doi.org/10.1117/12.2079803
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Despite recent findings on the possible role of age-related cerebral microvasculature changes in cognition decline, previous studies of capillary blood flow in aging (using animal models) are scarce and limited to anesthetized conditions. Since anesthesia can have different effects in young and old animals, it may introduce a confounding effect in aging studies. The present study aimed to eliminate the potential confound introduced by anesthesia by measuring capillary blood flow parameters in both awake conditions and under isoflurane anesthesia. We used 2-photon laser scanning fluorescence microscopy to measure capillary diameter, red blood cell velocity and flux, hematocrit and capillary volumetric flow in individual capillaries in the barrel cortex of 6- and 24-month old C57Bl/6 mice. It was observed that microvascular properties are significantly affected by anesthesia leading to different trends in capillary blood flow parameters with aging when measured under awake or anesthetized conditions. The findings in this study suggest taking extra care in interpreting aging studies from anesthetized animals.

Citation Download Citation

Mohammad Moeini, Maryam S. Tabatabaei, Samuel Bélanger, Pramod Avti, Alexandre Castonguay, Philippe Pouliot, and Frédéric Lesage "Effects of anesthesia on the cerebral capillary blood flow in young and old mice", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 932929 (5 March 2015); https://doi.org/10.1117/12.2079803

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