Motionless polarization-resolved second harmonic generation imaging of corneal collagen

Hans Georg Breunig; Ana Batista; Aisada Uchugonova; Karsten König

doi:10.1117/12.2078701

5 March 2015 Motionless polarization-resolved second harmonic generation imaging of corneal collagen

Hans Georg Breunig, Ana Batista, Aisada Uchugonova, Karsten König

Proceedings Volume 9329, Multiphoton Microscopy in the Biomedical Sciences XV; 93292P (2015) https://doi.org/10.1117/12.2078701
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Polarization-resolved second harmonic generation microscopy was used to investigate the collagenous structures of cornea samples in vitro in forward and backward direction. Although structural features appear different in both directions, following an approach by Latour et al. the collagen domain orientation is determined in forward as well as in backward direction, the latter being the only accessible direction for in vivo imaging. The experimental setup enables fast and completely motionless rotation of the polarization direction of 100 fs pulses by a polarization rotation based on a liquid crystal retarder.

Citation Download Citation

Hans Georg Breunig, Ana Batista, Aisada Uchugonova, and Karsten König "Motionless polarization-resolved second harmonic generation imaging of corneal collagen ", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93292P (5 March 2015); https://doi.org/10.1117/12.2078701

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