H4 tail interactions revealed by fluorescent fluctuation spectroscopy

Nathan Nurse; Chongli Yuan

doi:10.1117/12.2079810

9 March 2015 H4 tail interactions revealed by fluorescent fluctuation spectroscopy

Nathan Nurse, Chongli Yuan

Proceedings Volume 9331, Single Molecule Spectroscopy and Superresolution Imaging VIII; 933104 (2015) https://doi.org/10.1117/12.2079810
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Post-translational modifications to histone tails nave been shown to play a large role in dictating the conformation of chromatin. Structural changes in chromatin can play a large role in gene expression as compact chromatin can occlude transcriptional machinery. The role of the flexible regions of H4 N terminal tails is investigated using fluorescent correlation spectroscopy and the photon counting histogram. The combination of these techniques allows for the distinction between intra-array and inter-array interactions, as well as reveals structural changes that result from these interactions. The H4 tail was found to partake in attractive intra-array interactions that compact the 6x167 nucleosome arrays but did not partake in inter-array interactions that lead to oligomerization.

Citation Download Citation

Nathan Nurse and Chongli Yuan "H4 tail interactions revealed by fluorescent fluctuation spectroscopy", Proc. SPIE 9331, Single Molecule Spectroscopy and Superresolution Imaging VIII, 933104 (9 March 2015); https://doi.org/10.1117/12.2079810

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