Realization of super-resolution imaging by microlens-assisted laser scanning microscopy

Kuan-Yu Li; Yun-Ju Liu; Zong-Xing Lou; Yang Tsao; Kung-Hsuan Lin; Chih-Wei Chang; Shi-Wei Chu

doi:10.1117/12.2078060

9 March 2015 Realization of super-resolution imaging by microlens-assisted laser scanning microscopy

Kuan-Yu Li, Yun-Ju Liu, Zong-Xing Lou, Yang Tsao, Kung-Hsuan Lin, Chih-Wei Chang, Shi-Wei Chu

Proceedings Volume 9331, Single Molecule Spectroscopy and Superresolution Imaging VIII; 93310O (2015) https://doi.org/10.1117/12.2078060
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

In the field of optical microscopy, it is well known that spatial resolution is limited by diffraction of light. There have been variously efforts to achieve resolution beyond diffraction limit. However, most previous methods rely on nonlinearities of fluorescence, and thus require high-intensity lasers or special labeling strategies. In 2011, a novel superresolution technique based on a dielectric microlens and simple bright field microscope was demonstrated. The scheme is unexpectedly simple, since neither labeling nor intense laser is necessary, while the resolution is significantly higher than diffraction limit. Nevertheless, the contrast of bright field microscope is poor. In this work, we combine a dielectric microlens along with confocal laser scanning microscopy to considerably enhance image contrast. By simply inserting a microsphere onto the sample, the resolution is undoubtedly better than diffraction limit of the objective. Meanwhile, the contrast exhibits almost one order of enhancement. Field of view and magnification of the microlens imaging system are also characterized. Comparing with bright field microscope, laser scanning confocal microscopy provides better contrast under this microslens assisted super-resolution scheme. Our finding will contribute to material science and biomedicine research.

Citation Download Citation

Kuan-Yu Li, Yun-Ju Liu, Zong-Xing Lou, Yang Tsao, Kung-Hsuan Lin, Chih-Wei Chang, and Shi-Wei Chu "Realization of super-resolution imaging by microlens-assisted laser scanning microscopy", Proc. SPIE 9331, Single Molecule Spectroscopy and Superresolution Imaging VIII, 93310O (9 March 2015); https://doi.org/10.1117/12.2078060

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KEYWORDS

Microlens

Super resolution

Diffraction

Microscopes

Microscopy

Dielectrics

Gold

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