Multi-mode microscopy in real-time with LED array illumination

Ziji Liu; Lei Tian; Laura Waller

doi:10.1117/12.2075951

11 March 2015 Multi-mode microscopy in real-time with LED array illumination

Ziji Liu, Lei Tian, Laura Waller

Proceedings Volume 9336, Quantitative Phase Imaging; 93362M (2015) https://doi.org/10.1117/12.2075951
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Brightﬁeld, darkﬁeld and phase contrast are three of the most popular imaging modalities in biological microscopy. Each provides visualization of diﬀerent sample properties, without ﬂuorescent labels or staining. We demonstrate here a single-camera imaging system that can simultaneously acquire brightﬁeld, darkﬁeld and phase contrast images in real-time. Our method uses a programmable LED array as the illumination source, which provides ﬂexible patterning of illumination angles. We achieve a frame rate of 50 Hz with 2560×2160 pixels of lateral resolution, with speed being limited by the camera.

Citation Download Citation

Ziji Liu, Lei Tian, and Laura Waller "Multi-mode microscopy in real-time with LED array illumination", Proc. SPIE 9336, Quantitative Phase Imaging, 93362M (11 March 2015); https://doi.org/10.1117/12.2075951

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