Nano-imaging collagen by atomic force, near-field and nonlinear microscope

Ken Choong Lim; Jinkai Tang; Hao Li; Boon Ping Ng; Shaw Wei Kok; Qijie Wang; Ying Zhang

doi:10.1117/12.2078911

11 March 2015 Nano-imaging collagen by atomic force, near-field and nonlinear microscope

Ken Choong Lim, Jinkai Tang, Hao Li, Boon Ping Ng, Shaw Wei Kok, Qijie Wang, Ying Zhang

Proceedings Volume 9337, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII; 933709 (2015) https://doi.org/10.1117/12.2078911
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

As the most abundant protein in the human body, collagen has a very important role in vast numbers of bio-medical applications. The unique second order nonlinear properties of fibrillar collagen make it a very important index in nonlinear optical imaging based disease diagnosis of the brain, skin, liver, colon, kidney, bone, heart and other organs in the human body. The second-order nonlinear susceptibility of collagen has been explored at the macroscopic level and was explained as a volume-averaged molecular hyperpolarizability. However, details about the origin of optical second harmonic signals from collagen fibrils at the molecular level are still not clear. Such information is necessary for accurate interpolation of bio-information from nonlinear optical imaging techniques. The later has shown great potential in collagen based disease diagnosis methodologies. In this paper, we report our work using an atomic force microscope (AFM), near field (SNOM) and nonlinear laser scanning microscope (NLSM) to study the structure of collagen fibrils and other pro-collagen structures.

Citation Download Citation

Ken Choong Lim, Jinkai Tang, Hao Li, Boon Ping Ng, Shaw Wei Kok, Qijie Wang, and Ying Zhang "Nano-imaging collagen by atomic force, near-field and nonlinear microscope", Proc. SPIE 9337, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII, 933709 (11 March 2015); https://doi.org/10.1117/12.2078911

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available