Improvement of spatial resolution in confocal microscope with shifted-focus phase filter

Xiangdong Huang; Xiaoyan Xiang; Chongyang Wang

doi:10.1117/12.2181493

6 March 2015 Improvement of spatial resolution in confocal microscope with shifted-focus phase filter

Xiangdong Huang, Xiaoyan Xiang, Chongyang Wang

Proceedings Volume 9446, Ninth International Symposium on Precision Engineering Measurement and Instrumentation; 94463U (2015) https://doi.org/10.1117/12.2181493
Event: International Symposium on Precision Engineering Measurement and Instrumentation, 2014, Changsha/Zhangjiajie, China

Abstract

A spatial super-resolution method is proposed based on the multiplicative character of confocal microscope’s amplitude point-spread functions. The axial resolution can be greatly improved by introducing a shifted-focus phase filters in illumination part of a confocal microscope. However, this improvement is accompanied by a decrease of transversal resolution. Thus, a super-Gaussian phase filter is optimized to control the focal shift and transversal intensity distribution in a confocal microscope. Numerical simulation results indicate that the proposed method is useful to obtain a significant improvement in the optical sectioning capacity.

Citation Download Citation

Xiangdong Huang, Xiaoyan Xiang, and Chongyang Wang "Improvement of spatial resolution in confocal microscope with shifted-focus phase filter", Proc. SPIE 9446, Ninth International Symposium on Precision Engineering Measurement and Instrumentation, 94463U (6 March 2015); https://doi.org/10.1117/12.2181493

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