Bioaerosol detection and classification using dual excitation wavelength laser-induced fluorescence

Per Jonsson; Pär Wästerby; Per-Åke Gradmark; Julia Hedborg; Anders Larsson; Lars Landström

doi:10.1117/12.2176744

22 May 2015 Bioaerosol detection and classification using dual excitation wavelength laser-induced fluorescence

Per Jonsson, Pär Wästerby, Per-Åke Gradmark, Julia Hedborg, Anders Larsson, Lars Landström

Proceedings Volume 9455, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVI; 945509 (2015) https://doi.org/10.1117/12.2176744
Event: SPIE Defense + Security, 2015, Baltimore, MD, United States

Abstract

We present results obtained by a detection system designed to measure laser-induced fluorescence from individual aerosol particles using dual excitation wavelengths. The aerosol is sampled from ambient air and via a 1 mm diameter nozzle, surrounded by a sheath air flow, confined into a particle beam. A continuous wave blue laser at 404 nm is focused on the aerosol beam and two photomultiplier tubes monitor the presence of individual particles by simultaneous measuring the scattered light and any induced fluorescence. When a particle is present in the detection volume, a laser pulse is triggered from an ultraviolet laser at 263 nm and the corresponding fluorescence spectrum is acquired with a spectrometer based on a diffraction grating and a 32 channel photomultiplier tube array with single-photon sensitivity. The spectrometer measures the fluorescence spectra in the wavelength region from 250 to 800 nm. In the present report, data were measured on different monodisperse reference aerosols, simulants of biological warfare agents, and different interference aerosol particles, e.g. pollen. In the analysis of the experimental data, i.e., the time-resolved scattered and fluorescence signals from 404 nm c.w. light excitation and the fluorescence spectra obtained by a pulsed 263 nm laser source, we use multivariate data analysis methods to classify each individual aerosol particle.

Citation Download Citation

Per Jonsson, Pär Wästerby, Per-Åke Gradmark, Julia Hedborg, Anders Larsson, and Lars Landström "Bioaerosol detection and classification using dual excitation wavelength laser-induced fluorescence", Proc. SPIE 9455, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVI, 945509 (22 May 2015); https://doi.org/10.1117/12.2176744

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