Nature-inspired magnetoelastic biosentinels for the detection of pathogenic bacteria in stagnant liquids

Shin Horikawa; Yating Chai; Howard C. Wikle III; Jing Dai; Jiajia Hu; Sang-Jin Suh; Vitaly Vodyanoy; Bryan A. Chin

doi:10.1117/12.2087766

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13 May 2015 Nature-inspired magnetoelastic biosentinels for the detection of pathogenic bacteria in stagnant liquids

Shin Horikawa, Yating Chai, Howard C. Wikle III, Jing Dai, Jiajia Hu, Sang-Jin Suh, Vitaly Vodyanoy, Bryan A. Chin

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Proceedings Volume 9488, Sensing for Agriculture and Food Quality and Safety VII; 94880C (2015) https://doi.org/10.1117/12.2087766
Event: SPIE Sensing Technology + Applications, 2015, Baltimore, Maryland, United States

Abstract

This paper presents an investigation into magnetoelastic (ME) biosentinels that capture and detect low-concentration pathogenic bacteria in stagnant liquids. The ME biosentinels are designed to mimic a variety of white blood cell types, known as the main defensive mechanism in the human body against different pathogenic invaders. The ME biosentinels are composed of a freestanding ME resonator coated with an engineered phage that specifically binds with the pathogens of interest. These biosentinels are ferromagnetic and thus can be moved through a liquid by externally applied magnetic fields. In addition, when a time-varying magnetic field is applied, the ME biosentinels can be placed into mechanical resonance by magnetostriction. As soon as the biosentinels bind with the target pathogen through the phage-based biomolecular recognition, a change in the biosentinel’s resonant frequency occurs, and thereby the presence of the target pathogen can be detected. Detection of Bacillus anthracis spores under stagnant flow conditions was demonstrated.

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Shin Horikawa, Yating Chai, Howard C. Wikle III, Jing Dai, Jiajia Hu, Sang-Jin Suh, Vitaly Vodyanoy, and Bryan A. Chin "Nature-inspired magnetoelastic biosentinels for the detection of pathogenic bacteria in stagnant liquids", Proc. SPIE 9488, Sensing for Agriculture and Food Quality and Safety VII, 94880C (13 May 2015); https://doi.org/10.1117/12.2087766

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