Hyperspectral light field imaging

Raimund Leitner; Andreas Kenda; Andreas Tortschanoff

doi:10.1117/12.2179463

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5 May 2015 Hyperspectral light field imaging

Raimund Leitner, Andreas Kenda, Andreas Tortschanoff

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Proceedings Volume 9506, Optical Sensors 2015; 950605 (2015) https://doi.org/10.1117/12.2179463
Event: SPIE Optics + Optoelectronics, 2015, Prague, Czech Republic

Abstract

A light field camera acquires the intensity and direction of rays from a scene providing a 4D representation L(x,y,u,v) called the light field. The acquired light field allows to virtually change view point and selectively re-focus regions algorithmically, an important feature for many applications in imaging and microscopy. The combination with hyperspectral imaging provides the additional advantage that small objects (beads, cells, nuclei) can be categorised using their spectroscopic signatures. Using an inverse fluorescence microscope, a LCTF tuneable filter and a light field setup as a test-bed, fluorescence-marked beads have been imaged and reconstructed into a 4D hyper-spectral image cube L_HSI(x,y,z,λ). The results demonstrate the advantages of the approach for fluorescence microscopy providing extended depth of focus (DoF) and the fidelity of hyper-spectral imaging.

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Raimund Leitner, Andreas Kenda, and Andreas Tortschanoff "Hyperspectral light field imaging", Proc. SPIE 9506, Optical Sensors 2015, 950605 (5 May 2015); https://doi.org/10.1117/12.2179463

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